School personnel information




stem cell biology, eye development, regenerative medicine, pluripotent stem cells, epithelium, differentiation

Mail Address

Mail Address




Organization 【 display / non-display

  • 2010.07.01 - 2014.03.31, Graduate School of Medicine, Assistant Professor

  • 2014.04.01 - 2017.03.31, Graduate School of Medicine, Associate Professor

  • 2017.04.01 - , Graduate School of Medicine, Professor

Employment Record 【 display / non-display

Tohoku University Graduate School of Medicine 2008.07 - 2010.06
Osaka University Medical School 2010.07 -

Research topics 【 display / non-display

  • We are working on in vitro generation of ocular tissues using pluripotent stem cells (iPS, ES cells) and successfully generated 2-dimensional "whole eye organoids" from human pluripotent stem cells (Nature 2016). Furthermore, we performed its application to ocular regenerative medicine such as a corneal regeneration.
    Ophthalmology-related, Cell biology-related, Developmental biology-related, Molecular biology-related

  • We have been working on the regulation mechanism of cell development and differentiation by ECM-mediated signalling. Particularly focus on laminin isoforms and YAP-mediated signal transduction.
    Cell biology-related, Molecular biology-related


Academic Papers 【 display / non-display

  • The secretome of adipose-derived mesenchymal stem cells attenuates epithelial-mesenchymal transition in human corneal epithelium., Shibata S, Hayashi R, Okubo T, Kudo Y, Baba K, Honma Y, Nishida K. , Regen Ther.,2(11) 114-122, 2019.07, Papers

  • CD200 facilitates the isolation of corneal epithelial cells derived from human pluripotent stem cells., Hayashi R, Ishikawa Y, Katayama T, Quantock AJ, Nishida K, Scientific reports,8(1):16550, 2018.11, Papers

  • Selective laminin-directed differentiation of human induced pluripotent stem cells into distinct ocular lineages., Shibata, S., Hayashi, R., Okubo, T., Kudo, Y., Katayama, T., Ishikawa, Y., Toga, J., Yagi, E., Honma, Y., Quantock, A.J., Sekiguchi, K., and Nishida, K., Cell Rep.,25(6): 1668-1679, 2018.11, Papers

  • A new in vitro model of gelatinous drop-like corneal dystrophy by knocking out TACSTD2 and its paralogous gene EpCAM in human corneal epithelial cells., Xu P, Kai C, Kobayashi Y, Yamamoto Y, Tsujikawa M, Hayashi R, and Nishida K. , Transl Vis Sci Technol. ,7(6) 30, 2018.11, Papers

  • Use of homeobox gene expression patterns to determine anatomical regions of origin for body surface tissues derived from adult mice., Okubo T, Hayashi R, Shibata S, Kudo Y, Honma Y, Nishida K., J Tissue Eng Regen Med.,Jun;12(6):1412-1419., 2018.06, Papers

display all >>

Books 【 display / non-display

  • General, Epithelail stem cell, Ryuhei Hayashi, 2011.07

Awards 【 display / non-display

  • Japanese Ophthalmological Society Young Investigator Award, Ryuhei Hayashi, Japanese Ophthalmological Society, 2018.04

  • Japan Cornea Society Young Investigator Award, Ryuhei Hayashi, Japan Cornea Society, 2017.02

  • Osaka University Presidential Awards for Encouragement, Ryuhei Hayashi, Osaka University, 2015.07

  • Charitable Trust Fund for Ophthalmic Research in Commemoration of Santen Pharmaceutical's Founder, Ryuhei Hayashi, Charitable Trust Fund for Ophthalmic Research in Commemoration of Santen Pharmaceutical's Founder, 2011.10

  • 1st Wakamoto Conference of Advanced Medicine of Ophthalmology in Tokyo; WACAMOTO GOLD AWARD, Ryuhei Hayashi, Wakamoto Pharmaceutical, 2011.07

display all >>

Media report 【 display / non-display

  • Eye-Opening Study Differentiates iPS Cells into Various Ocular Lineages, Osaka University, 2018.12

  • Researchers Develop 2D `Eye' From Induced Pluripotent Stem Cells, Asian Scientist Magazine, 2016.06

  • Mimicking the development of the whole eye with human iPS cells, ScienceDaily, 2016.05