School personnel information


TAKAO Toshifumi


Protein Chemistry, Mass Spectrometry, Proteomics

Mail Address

Mail Address




Organization 【 display / non-display

  • 2002.03.01 - , Institute for Protein Research, Professor

Education 【 display / non-display

Osaka University Faculty of Science  Graduated 1981.03
Osaka University Graduate School, Division of Natural Science  Completed Ph.D. 1986.12

Research topics 【 display / non-display

  • Mass spectrometry is well accepted technique for the analyses of chemical structures of biological compounds. For the last two decades, we have been working to develop methods for determining primary structures and post-translational modifications of proteins by using mass spectrometry.
    Structural biochemistry-related


Academic Papers 【 display / non-display

  • Quantitative Analysis of Deamidation and Isomerization in β2-Microglobulin by 18O Labeling, Masafumi Fukuda and Toshifumi Takao, Analytical Chemistry,84, 10388-10394, 2012.11, Papers

  • Lithium ion adduction enables UPLC-MS/MS-based analysis of multi-class, 3-hydroxyl group-containing keto-steroids., Wang Q, Shimizu K, Maehata K, Pan Y, Sakurai K, Hikida T, Fukada Y, Takao T., The Journal of Lipid Research, 2020.02, Papers

  • The phosphorylation of sorting nexin 5 at serine 226 regulates retrograde transport and macropinocytosis, Itai N, Shimazu T, Kimura T, Ibe I, Yamashita R, Kaburagi Y, Dohi T, Tonozuka T, Takao T, Nishikawa A, PLoS One (2018),12, 2018.11, Papers

  • The zinc form of carnosine dipeptidase 2 (CN2) has dipeptidase activity but its substrate specificity is different from that of the manganese form., Okumura N, Takao T, Biochemical and Biophysical Research Communications, 2017.12, Papers

  • One-Pot Four-Segment Ligation Using Seleno- and Thioesters: Synthesis of Superoxide Dismutase, T. Takei, T. Andoh, T. Takao, H. Hojo, Angewandte Chemie International Edition,56, 49, 15708-15711, 2017.12, Papers

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Books 【 display / non-display

  • Special, Peptide disease markers from urine, Kouzou SUTO, Yoshinori SATOMI, Toshifumi TAKAO, 2007.06

  • Other, Methods for Determination of Protein Sequences by Fast Atom Bombardment Mass Spectrometry in Laboratory Methodology in Biochemistry, 1990

Patents / Utility models / Designs 【 display / non-display

  • Japan, A method and material for separation of amino-terminally blocked peptides, Toshiyuki MIKAMI, Toshifumi TAKAO, 特許第5415000号(Registration), 2008.02, 2013.11

  • 日本 欧州, A method to diagnose lung adenocarcinoma , Masamitsu NAKAZATO, Toshifumi TAKAO, 特許第6613490号(Registration), 2017.02, 2019.11

  • Japan, Bioactive peptides, Motoo YAMASAKI, Noriyuki TAKAHASHI, Naoto MINAMINO, Kazuki SASAKI, Toshifumi TAKAO, Yoshinori SATOMI, PCT/JP2006/314969号(Publication), 2006.12

  • Japan, Bioactive polypeptides and applications, Masamitsu NAKAZATO, Toshifumi TAKAO, PCT/JP2006/309192号(Publication), 2006.12