School personnel information




Archaea, DNA repair

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Mail Address

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Organization 【 display / non-display

  • 2017.09.01 - , Department of Materials Engineering Science, Graduate School of Engineering Science, Assistant Professor

Education 【 display / non-display

Kyushu University  Department of Bioscience and Biotechnology Completed Ph.D. (Agriculture) 2017.03
Kyushu University   Department of Bioscience and Biotechnology Completed 2014.03
Kyushu University Faculty of Agriculture Department of Bioresource and Bioenvironment Graduated 2012.03

Employment Record 【 display / non-display

Kyushu Universiry 2017.04 - 2017.08
University of Illinois at Urbana- Champaign, Visiting scholar 2014.05 - 2016.12
Grant-in-Aid for Japan Society for the Promotion of Science Fellow 2014.04 - 2017.03

Research topics 【 display / non-display

  • Endonuclease Q (EndoQ) is a recently discovered enzyme from the hyperthermophilic archaeon Pyrococcus furiosus by Ishino's group. EndoQ can specifically recognize and cut the DNA backbone at the 5'-side of deaminated bases (uracil, hypoxanthine, xnathine) and abasic site. EndoQ was also found to interact with PCNA, a sliding clamp, in P. furiosus, and thus, EndoQ has been considered as a DNA repair enzyme. However, the repair pathway madiated by EndoQ is still little understood; e.g. which polymerase is involved, how to remove the lesion. In this study, we address the fully understanding of the EndoQ-mediated DNA repair pathway by identifying the interacting partner of EndoQ from P. furiosus.
    Applied biochemistry-related, Molecular biology-related

Academic Society Membership 【 display / non-display

  • The Molecular Biology Society of Japan

  • Japan Society for Bioscience, Biotechnology, and Agrochemistry


Academic Papers 【 display / non-display

  • The mesophilic archaeon Methanosarcina acetivorans counteracts uracil in DNA with multiple enzymes: EndoQ, ExoIII, and UDG., Shiraishi M, Ishino S, Heffernan M, Cann I, Ishino Y, Scientific reports,8(1) 15791, 2018.10, Other

  • Fluorescence detection of DNA mismatch repair in human cells, Shunsuke Ito, Miyako Shiraishi, Kazuki Tsuchihashi, Reine Takatsuka, Junpei Yamamoto, Isao Kuraoka, Shigenori Iwai, Scientific Reports,8(1) 12181, 2018.08, Papers

  • A functional endonuclease Q exists in the bacterial domain: identification and characterization of endonuclease Q from Bacillus pumilus, Miyako Shiraishi, Sonoko Ishino, Isaac Cann, Yoshizumi Ishino, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,81(5) 931-937, 2017.05, Papers

  • PCNA is involved in the EndoQ-mediated DNA repair process in Thermococcales, Miyako Shiraishi, Sonoko Ishino, Kotaro Yoshida, Takeshi Yamagami, Isaac Cann, Yoshizumi Ishino, SCIENTIFIC REPORTS,6 25532, 2016.05, Papers

  • EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furiosus, Sonoko Ishino, Naruto Makita, Miyako Shiraishi, Takeshi Yamagami, Yoshizumi Ishino, BIOCHIMIE,118 264-269, 2015.11, Papers

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Patents / Utility models / Designs 【 display / non-display

  • United States, Novel DNA cleavage enzyme, Ishino Y. Ishino S. Shiraishi M., 14/890,009(Application), 2013.05

Awards 【 display / non-display

  • The International Society for Extremophiles Poster Award, Miyako Shiraishi, The International Society for Extremophile, 2016.09