School personnel information


Miyanoiri Youhei


Structural Biology, NMR

Laboratory Phone number





Organization 【 display / non-display

  • 2017.04.01 - , Institute for Protein Research, Associate Professor

Education 【 display / non-display

Yokohama National University   Completed Ph.D 2005.03

Research topics 【 display / non-display

  • Our research is concerned with structural and dynamical aspects of protein function. We are interested in elucidating correlation between structure, dynamics and biological functions of macromolecular proteins. To pursue our goals, we use nuclear magnetic resonance spectroscopy (NMR). The NMR method targets solution proteins under various conditions, included in living cells. Therefore, it allows us to obtain crucial information for elucidating biological functions of proteins at an atomic resolution. Although NMR is powerful tool, its potential has been limited due to the presence of signal broadening, overlapping and lower sensitivity in large molecular weight proteins (ex. > 25 kDa). To overcome this problem, various isotope labeling techniques were developed. Our individual techniques, Stereo array isotope labeling (SAIL) method contributed significantly to improve NMR spectra of larger proteins and succeeded in determining precise structure of 42 kDa proteins. For much larger proteins and protein complexes (i.e. > 50 kDa), presently, we use site specific amide (15NH) and methyl (13CH3) labeling methods. Although it allows us to obtain highly sensitive methyl and amide TROSY signals that enable the study of local structure, it's still not sufficient for NMR-based structure determination which is needed for understanding precise protein function. In order to solve this problem, we have been developing the new SAIL amino acids. Our new SAIL methods allow us to observe the several 1H-13C signals even in 80-1000 kDa large molecular proteins. This information is very useful for understanding the precise structure and dynamic property of various macromolecular proteins such as membrane proteins and protein-protein complexes.
    Structural biochemistry-related


Academic Papers 【 display / non-display

  • Recent Developments in Isotope-aided NMR Methods for Supramolecular Protein Complexes -SAIL Aromatic TROSY, Miyanoiri Y, Takeda M, Terauchi T, Kainosho M, , Biochimica et Biophysica Acta - General Subjects,1864・2, 2019.10,, Papers

  • Dynamic Views of the Fc Region of Immunoglobulin G Provided by Experimental and Computational Observations. , Yanaka S, Yogo R, Inoue R, Sugiyama M, Itoh SG, Okumura H, Miyanoiri Y,Yagi H, Satoh T, Yamaguchi T, Kato K, Antibodies,8・3・39, 2019.07, Papers

  • Aromatic Ring Dynamics, Thermal Activation, and Transient Conformations of a 468 kDa Enzyme by Specific 1H-13C Labeling and Fast Magic-Angle Spinning NMR., Gauto DF, Macek P, Barducci A, Fraga H, Hessel A, Terauchi T, Gajan D, Miyanoiri Y,Boisbouvier J, Lichtenecker R, Kainosho M, Schanda P, Journal of the American Chemical Society,141・28・11183-11195, 2019.06, Papers

  • Dynamic Structure of C-terminal Domain of FliG Revealed by NMR and MD Simulation Analysis, Miyanoiri Y, Hijikata A, Homma M, SEIBUTSU BUTSURI,58・5・251-254, 2018.10, Papers(In Japanese)

  • Evolution and diversification of the plant gibberellin receptor GID1. , Yoshida H, Tanimoto E, Hirai T, Miyanoiri Y, Mitani R, Kawamura M, Takeda M,  Takehara S, Hirano K, Kainosho M, Akagi T, Matsuoka M, Ueguchi-Tanaka M , Proceedings of the National Academy of Sciences of the United States of America,115・33・E7844-E7853, 2018.08, Papers

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Books 【 display / non-display

  • Special, New SAIL-NMR method for structural analysis of large molecular proteins, Miyanoiri Y, Takeda M, Terauchi T, Kainosho M, Hokuryukan & NEW SCIENCE co., ltd. , ISBN, 04128-10, 2019.09


Conference management 【 display / non-display

  • International Conference, The XXXVIIth International Conference on Magnetic Resonance in Biological Systems, Executive Committee, 2016.08

  • International Conference, WPI-next International Workshop -High Resolution Cell Biology-, 2015.10

  • International Conference, IPR seminar -Practical Aspects of Non-uniform Sampling in Multi-dimensional NMR Spectroscopy and Application for Biological System, 2014.06